What if you have a need for liquid nitrogen, but you do not wish to simply order it from a local supplier? In that case you can build your very own pulse tube cryocooler, as [Hyperspace Pirate] is ...
Keep the tissue frozen by the addition of liquid nitrogen. iii Transfer the powdered tissue to a polypropylene snap-cap tube containing 3 ml of solution D and homogenize at 15–25 °C for 15–30 ...
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