Rev IT AAV Enhancer increased genome titers 1.7- to 2.2-fold with other transfection reagents compared to their respective controls, demonstrating broad applicability. Figure 1.

This schematic shows the steps involved in generating recombinant AAV by the triple-transfection method using plasmid DNA. The application of the cell-based qPCR assay at SK pharmteco has proven ...

AAV is manufactured by the triple transfection of three plasmid DNA constructs into packaging cell lines to produce recombinant adeno-associated virus.

Material and methods A commercial vendor received two AAV samples, one serotype 5 (AAV5) and one serotype 9 (AAV9). The two samples were generated via the triple plasmid transfection approach.

In this talk, we present optimization strategies for the transfection step of vector production to generate high titers of both AAV and LV using TransIT-VirusGEN® Transfection Reagent. The VirusGEN® ...

Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show great promise for treatment of diseases from genetic disorders to cancer.

TruStable™ production cell line produced ~15-fold more AAV with ~3-fold higher packaging percentage than the comparator triple transfection system, resulting in ~30-fold more material generated ...

Agathos records first sale of recombinant adeno-associated virus produced using AE1-BHK to collaborator Genovac; provides free access to AE1-BHK cell line ...

But the triple transfection of the required plasmids, and in the strict stoichiometries required for AAV assembly, we believe adds enormously to the cost of traditional AAV manufacturing, limiting ...